UNIT 2.15 Isolation of Single-Stranded DNA
Published Online: 1 JUL 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Wakimoto, Y., Jiang, J. and Wakimoto, H. 2014. Isolation of Single-Stranded DNA. Current Protocols in Molecular Biology. 107:VI:2.15:2.15.1–2.15.9.
- Published Online: 1 JUL 2014
Single-stranded DNA (ssDNA) is often used for DNA sequencing as well as microarray and hybridization technologies. Asymmetric PCR or exonuclease digestion followed by urea gel separation and isolation on streptavidin-coated magnetic beads are commonly used for this purpose. These two methods may not yield large amounts of highly purified ssDNA. This protocol for ssDNA isolation from PCR-amplified DNA involves biotin labeling of one strand and subsequent strand separation utilizing streptavidin-coated magnetic beads. Curr. Protoc. Mol. Biol. 107:2.15.1-2.15.9. © 2014 by John Wiley & Sons, Inc.
- single-stranded DNA;
- magnetic beads