UNIT 3.2 Mapping by Multiple Endonuclease Digestions
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Bloch, K. D. 2001. Mapping by Multiple Endonuclease Digestions. Current Protocols in Molecular Biology. 13:II:3.2:3.2.1–3.2.5.
- Published Online: 1 MAY 2001
- Published Print: JAN 1991
In this unit, a protocol is provided in which the DNA of interest is cleaved with a variety of restriction endonucleases, either individually or in combination, and the resulting products are separated by agarose or acrylamide gel electrophoresis. By determining the sizes of DNA fragments produced by endonuclease cleavage, the restriction map is deduced progressively from simple situations where enzymes cleave the DNA once or twice to more complex situations where cleavage occurs more frequently.