Unit

UNIT 3.2 Mapping by Multiple Endonuclease Digestions

  1. Kenneth D. Bloch

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0302s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Bloch, K. D. 2001. Mapping by Multiple Endonuclease Digestions. Current Protocols in Molecular Biology. 13:II:3.2:3.2.1–3.2.5.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1991

Abstract

In this unit, a protocol is provided in which the DNA of interest is cleaved with a variety of restriction endonucleases, either individually or in combination, and the resulting products are separated by agarose or acrylamide gel electrophoresis. By determining the sizes of DNA fragments produced by endonuclease cleavage, the restriction map is deduced progressively from simple situations where enzymes cleave the DNA once or twice to more complex situations where cleavage occurs more frequently.