UNIT 3.3 Mapping by Partial Endonuclease Digestions
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Bloch, K. D. 2001. Mapping by Partial Endonuclease Digestions. Current Protocols in Molecular Biology. 00:II:3.3:3.3.1–3.3.2.
- Published Online: 1 MAY 2001
- Published Print: JAN 1990
In the procedure described in this unit, a DNA fragment radiolabeled at one of its two ends is purified by gel electrophoresis and subjected to partial cleavage by a restriction endonuclease. Analysis of the resulting products by polyacrylamide (or agarose) gel electrophoresis enables one to define the distance of restriction sites from the labeled end.