Unit

UNIT 3.3 Mapping by Partial Endonuclease Digestions

  1. Kenneth D. Bloch

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0303s09

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Bloch, K. D. 2001. Mapping by Partial Endonuclease Digestions. Current Protocols in Molecular Biology. 00:II:3.3:3.3.1–3.3.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1990

Abstract

In the procedure described in this unit, a DNA fragment radiolabeled at one of its two ends is purified by gel electrophoresis and subjected to partial cleavage by a restriction endonuclease. Analysis of the resulting products by polyacrylamide (or agarose) gel electrophoresis enables one to define the distance of restriction sites from the labeled end.