UNIT 3.5 DNA-Dependent DNA Polymerases

  1. Rebecca B. Kucera,
  2. Nicole M. Nichols

Published Online: 1 OCT 2008

DOI: 10.1002/0471142727.mb0305s84

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Kucera, R. B. and Nichols, N. M. 2008. DNA-Dependent DNA Polymerases. Current Protocols in Molecular Biology. 84:III:3.5:3.5.1–3.5.19.

Author Information

  1. New England Biolabs, Ipswich, Massachusetts

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008


This unit presents characteristics and reaction conditions of the DNA-dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic DNA polymerases used in PCR applications (described fully in unit 15.1), and the rapidly expanding class of lesion-bypass DNA polymerases that play a role in DNA damage repair. Curr. Protoc. Mol. Biol. 84:3.5.1-3.5.19. © 2008 by John Wiley & Sons, Inc.


  • nick translation;
  • 3′-end labeling;
  • blunting ends;
  • random-primer synthesis;
  • whole genome amplification;
  • PCR;
  • lesion-bypass