UNIT 3.6 Template-Independent DNA Polymerases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Tabor, S. 2001. Template-Independent DNA Polymerases. Current Protocols in Molecular Biology. 00:III:3.6:3.6.1–3.6.2.
- Published Online: 1 MAY 2001
- Published Print: OCT 1987
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Terminal deoxynucleotidyl transferase, purified from calf thymus, catalyzes the incorporation of deoxynucleotides to the 3′-hydroxyl termini of DNA accompanied by the release of inorganic phosphate. Reaction conditions are described in this unit in addition to some applications, including cloning DNA fragments, labeling the 3′ termini of DNA with 32P, incorporating nonradioactive tags onto the 3′ termini of DNA fragments, and synthesizing model polydeoxynucleotide homopolymers.