Unit

UNIT 3.6 Template-Independent DNA Polymerases

  1. Stanley Tabor

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0306s00

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tabor, S. 2001. Template-Independent DNA Polymerases. Current Protocols in Molecular Biology. 00:III:3.6:3.6.1–3.6.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1987

This is not the most recent version of the article. View current version (1 OCT 2008)

Abstract

Terminal deoxynucleotidyl transferase, purified from calf thymus, catalyzes the incorporation of deoxynucleotides to the 3′-hydroxyl termini of DNA accompanied by the release of inorganic phosphate. Reaction conditions are described in this unit in addition to some applications, including cloning DNA fragments, labeling the 3′ termini of DNA with 32P, incorporating nonradioactive tags onto the 3′ termini of DNA fragments, and synthesizing model polydeoxynucleotide homopolymers.