UNIT 3.6 Template-Independent DNA Polymerases
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Yue, D., Tabor, S. and Nichols, N. M. 2008. Template-Independent DNA Polymerases. Current Protocols in Molecular Biology. 84:III:3.6:3.6.1–3.6.3.
- Published Online: 1 OCT 2008
- Published Print: OCT 2008
Terminal deoxynucleotidyl transferase (TdT), is a template-independent DNA polymerase that catalyzes the incorporation of deoxynucleotides at the 3′-hydroxyl terminus of DNA, accompanied by the release of inorganic phosphate. TdT does not require a template and will not copy one. Reaction conditions and some applications are described in this unit, including cloning DNA fragments, labeling the 3′ terminus of DNA with 32P or nonradioactive tags, synthesizing model polydeoxynucleotide homopolymers, and detecting DNA damage and apoptotic cells. Curr. Protoc. Mol. Biol. 84:3.6.1-3.6.3. © 2008 by John Wiley & Sons, Inc.
- terminal deoxynucleotidyl transferase;
- DNA 3′-end labeling;
- DNA tailing;
- TUNEL assay