Unit

UNIT 3.7 RNA-Dependent DNA Polymerases

  1. George Tzertzinis1,
  2. Stanley Tabor2,
  3. Nicole M. Nichols1

Published Online: 1 OCT 2008

DOI: 10.1002/0471142727.mb0307s84

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tzertzinis, G., Tabor, S. and Nichols, N. M. 2008. RNA-Dependent DNA Polymerases. Current Protocols in Molecular Biology. 84:3.7.1–3.7.4.

Author Information

  1. 1

    New England Biolabs, Ipswich, Massachusetts

  2. 2

    Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008

Abstract

Reverse transcriptases (RTs) are multifunctional enzymes, but are mainly used as RNA-directed DNA polymerases in first-strand cDNA synthesis. Specifically, oligodeoxynucleotides are used as primers for extension on RNA templates. The DNA synthesized from an RNA template is referred to as complementary DNA (cDNA) and is often used as a template for PCR or converted to dsDNA for cloning. This unit describes appropriate reaction conditions for RTs from Moloney murine leukemia virus (MMLV) and avian myeloblastosis virus (AMV), along with applications such as synthesizing cDNA, 3′ fill-in reactions, and labeling the 3′ terminus of DNA fragments with 5′ protruding ends, and DNA sequencing. Curr. Protoc. Mol. Biol. 84:3.7.1-3.7.4. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • first-strand cDNA;
  • RT-PCR;
  • MMLV RT;
  • AMV RT;
  • fill-in reaction