Unit

UNIT 3.8 DNA-Dependent RNA Polymerases

  1. Stanley Tabor

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0308s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tabor, S. 2001. DNA-Dependent RNA Polymerases. Current Protocols in Molecular Biology. 00:III:3.8:3.8.1–3.8.4.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1991

This is not the most recent version of the article. View current version (1 OCT 2008)

Abstract

There are two types of DNA-dependent RNA polymerases commonly used for nucleic acid research: E. coli RNA polymerase and the bacteriophage T7, T3, and SP6 RNA polymerases. This unit describes the uses and limitations of each class of RNA polymerase along with specific reaction conditions and applications.