UNIT 3.8 DNA-Dependent RNA Polymerases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Tabor, S. 2001. DNA-Dependent RNA Polymerases. Current Protocols in Molecular Biology. 00:3.8.1–3.8.4.
- Published Online: 1 MAY 2001
- Published Print: JAN 1991
This is not the most recent version of the article. View current version (1 OCT 2008)
There are two types of DNA-dependent RNA polymerases commonly used for nucleic acid research: E. coli RNA polymerase and the bacteriophage T7, T3, and SP6 RNA polymerases. This unit describes the uses and limitations of each class of RNA polymerase along with specific reaction conditions and applications.