Unit

UNIT 3.9 DNA-Independent RNA Polymerases

  1. Stanley Tabor

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0309s00

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tabor, S. 2001. DNA-Independent RNA Polymerases. Current Protocols in Molecular Biology. 00:III:3.9:3.9.1–3.9.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1987

This is not the most recent version of the article. View current version (1 OCT 2008)

Abstract

Poly(A) polyermase, purified from E. coli, catalyzes the incorporation of AMP residues onto the free 3′-hydroxyl terminus of RNA, utilizing ATP as a precursor. This unit describes specific reaction conditions as well as some applications including labeling the 3′ ends of RNA with α32ATP for hybridization probes, and cloning RNA that lacks a poly(A) tail. The poly(A) tail is synthesized by poly(A) polyermase, and cDNA is synthesized from the resulting RNA using oligo(dT) as a primer.