UNIT 3.9 DNA-Independent RNA Polymerases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Tabor, S. 2001. DNA-Independent RNA Polymerases. Current Protocols in Molecular Biology. 00:III:3.9:3.9.1–3.9.2.
- Published Online: 1 MAY 2001
- Published Print: OCT 1987
This is not the most recent version of the article. View current version (1 OCT 2008)
Poly(A) polyermase, purified from E. coli, catalyzes the incorporation of AMP residues onto the free 3′-hydroxyl terminus of RNA, utilizing ATP as a precursor. This unit describes specific reaction conditions as well as some applications including labeling the 3′ ends of RNA with α32ATP for hybridization probes, and cloning RNA that lacks a poly(A) tail. The poly(A) tail is synthesized by poly(A) polyermase, and cDNA is synthesized from the resulting RNA using oligo(dT) as a primer.