UNIT 3.9 DNA-Independent RNA Polymerases

  1. Stanley Tabor

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0309s00

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tabor, S. 2001. DNA-Independent RNA Polymerases. Current Protocols in Molecular Biology. 00:III:3.9:3.9.1–3.9.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1987

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Poly(A) polyermase, purified from E. coli, catalyzes the incorporation of AMP residues onto the free 3′-hydroxyl terminus of RNA, utilizing ATP as a precursor. This unit describes specific reaction conditions as well as some applications including labeling the 3′ ends of RNA with α32ATP for hybridization probes, and cloning RNA that lacks a poly(A) tail. The poly(A) tail is synthesized by poly(A) polyermase, and cDNA is synthesized from the resulting RNA using oligo(dT) as a primer.