UNIT 3.12 Endonucleases

Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also provided. For a detailed discussion of the endonucleases more typically associated with DNA damage repair (e.g., Endo III, IV, V and VIII of E. coli and human APE1), see UNIT 3.9. Curr. Protoc. Mol. Biol. 93:3.12.1-3.12.7. © 2011 by John Wiley & Sons, Inc.

Keywords: DNase I; mung bean nuclease; DNA fragmentation; next-generation DNA sequencing; micrococcal nuclease; MNase