UNIT 3.12 Endonucleases

  1. Nicole M. Nichols

Published Online: 1 JAN 2011

DOI: 10.1002/0471142727.mb0312s93

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Nichols, N. M. 2011. Endonucleases. Current Protocols in Molecular Biology. 93:III:3.12:3.12.1–3.12.7.

Author Information

  1. New England Biolabs, Inc., Ipswich, Massachusetts

Publication History

  1. Published Online: 1 JAN 2011
  2. Published Print: JAN 2011


Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also provided. For a detailed discussion of the endonucleases more typically associated with DNA damage repair (e.g., Endo III, IV, V and VIII of E. coli and human APE1), see unit 3.9. Curr. Protoc. Mol. Biol. 93:3.12.1-3.12.7. © 2011 by John Wiley & Sons, Inc.


  • DNase I;
  • mung bean nuclease;
  • DNA fragmentation;
  • next-generation DNA sequencing;
  • micrococcal nuclease;
  • MNase