UNIT 3.13 Ribonucleases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Struhl, K. 2001. Ribonucleases. Current Protocols in Molecular Biology. 8:III:3.13:3.13.1–3.13.3.
- Published Online: 1 MAY 2001
- Published Print: OCT 1989
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Ribonucleases (RNases) with different sequence specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. One very common application for RNase A is presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. Two other commonly used RNases, RNase H and RNase T1, are also described. In addition, many commercially available RNases are sequence-specific endoribonucleases, a property has been used for enzymatic sequencing of RNA.