UNIT 3.13 Ribonucleases
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Nichols, N. M. and Yue, D. 2008. Ribonucleases. Current Protocols in Molecular Biology. 84:III:3.13:3.13.1–3.13.8.
- Published Online: 1 OCT 2008
- Published Print: OCT 2008
Ribonucleases (RNases) with different sequence or structural specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. The development of RNase protection assays, structural determination assays, and the production of small interfering RNAs (siRNA) employed in RNA interference (RNAi) experiments has depended on the unique substrate specificities of commercially available RNases, including RNases A, I, T1, V1, HI, III, and Dicer. One very common application for high purity RNase A is also presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. RNase HII and the placental RNase inhibitor are also discussed. Curr. Protoc. Mol. Biol. 84:3.13.1-3.13.8. © 2008 by John Wiley & Sons, Inc.
- RNase protection assay;
- RNA footprinting;