Unit

UNIT 3.13 Ribonucleases

  1. Nicole M. Nichols,
  2. Dongxian Yue

Published Online: 1 OCT 2008

DOI: 10.1002/0471142727.mb0313s84

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Nichols, N. M. and Yue, D. 2008. Ribonucleases. Current Protocols in Molecular Biology. 84:III:3.13:3.13.1–3.13.8.

Author Information

  1. New England Biolabs, Ipswich, Massachusetts

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008

Abstract

Ribonucleases (RNases) with different sequence or structural specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. The development of RNase protection assays, structural determination assays, and the production of small interfering RNAs (siRNA) employed in RNA interference (RNAi) experiments has depended on the unique substrate specificities of commercially available RNases, including RNases A, I, T1, V1, HI, III, and Dicer. One very common application for high purity RNase A is also presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. RNase HII and the placental RNase inhibitor are also discussed. Curr. Protoc. Mol. Biol. 84:3.13.1-3.13.8. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • RNase;
  • RNase protection assay;
  • RNA footprinting;
  • RNAi;
  • siRNA