Unit

UNIT 3.15 RNA Ligases

  1. Stanley Tabor

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0315s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tabor, S. 2001. RNA Ligases. Current Protocols in Molecular Biology. 00:III:3.15:3.15.1–3.15.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 2000

This is not the most recent version of the article. View current version (1 OCT 2008)

Abstract

T4 RNA ligase, the product of the phage gene 63, is purified from phage-infected cells. It catalyzes the ATP-dependent covalent joining of single-stranded 5'-phosphoryl termini of DNA or RNA to single-stranded 3'-hydroxyl termini of DNA or RNA. This unit describes specific reaction conditions as well as applications such as radioactive labeling of 3' termini of RNA, circularizing deoxy- and ribo-oligonucleotides, ligating oligomers for oligonucleotide synthesis, and stimulating the blunt-end ligation activity of T4 DNA ligase.