UNIT 3.16 Subcloning of DNA Fragments

  1. Kevin Struhl

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0316s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Struhl, K. 2001. Subcloning of DNA Fragments. Current Protocols in Molecular Biology. 13:IV:3.16:3.16.1–3.16.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1991


The essence of recombinant DNA technology is the joining of two or more separate segments of DNA to generate a single DNA molecule that is capable of autonomous replication in a given host. The simplest constructions of hybrid DNA molecules involve the cloning of insert sequences into plasmid or bacteriophage cloning vectors. The insert sequences can derive from essentially any organism, and they may be isolated directly from the genome, from mRNA, or from previously cloned DNA segments (in which case, the procedure is termed subcloning). Alternatively, insert DNAs can be created directly by DNAsynthesis. This unit provides protocols for the subcloning of DNA fragments and ligation of DNA fragments in gels.