Unit

UNIT 3.17 Constructing Recombinant DNA Molecules by the Polymerase Chain Reaction

  1. Elaine A. Elion

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0317s24

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Elion, E. A. 2001. Constructing Recombinant DNA Molecules by the Polymerase Chain Reaction. Current Protocols in Molecular Biology. 3:IV:3.17.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1993

This is not the most recent version of the article. View current version (1 APR 2007)

Abstract

This unit describes the use of PCR to construct hybrid DNA molecules. The main objective is to give an overview of how PCR can be exploited to accomplish numerous cloning strategies; it is assumed that the reader is already familiar with basic molecular biology techniques including PCR amplification and subcloning. The Basic Protocol outlines the PCR amplification and cloning strategies. A troubleshooting guide for problems most frequently encountered in PCR cloning, and three specific examples of this technique--for creating (1) in-frame fusion proteins, (2) recombinant DNA products, and (3) deletions and inversions by inverse PCR--are also presented.