UNIT 3.17 Constructing Recombinant DNA Molecules by PCR
Published Online: 1 APR 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Elion, E. A., Marina, P. and Yu, L. 2007. Constructing Recombinant DNA Molecules by PCR. Current Protocols in Molecular Biology. 78:IV:3.17:3.17.1–3.17.12.
- Published Online: 1 APR 2007
- Published Print: APR 2007
This unit describes the use of PCR to construct hybrid DNA molecules. The unit provides an overview of how PCR can be exploited to accomplish numerous cloning strategies. The Basic Protocol outlines the PCR amplification and cloning strategies. The Commentary includes a troubleshooting guide for problems most frequently encountered in PCR cloning, plus four specific examples of the application of this technique to create in-frame fusion proteins, to create recombinant DNA products, to generate deletions and inversions by inverse PCR, and to introduce mutagenized PCR products or specific mutations or fusions by gap repair in yeast.
- recombinant DNA;
- fusion proteins