Unit

UNIT 3.20 Recombinational Cloning

  1. Jaehong Park,
  2. Joshua LaBaer

Published Online: 1 MAY 2006

DOI: 10.1002/0471142727.mb0320s74

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Park, J. and LaBaer, J. 2006. Recombinational Cloning. Current Protocols in Molecular Biology. 74:V:3.20:3.20.1–3.20.22.

Author Information

  1. Harvard Medical School, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2006
  2. Published Print: APR 2006

This is not the most recent version of the article. View current version (1 APR 2015)

Abstract

The study of protein en masse, or functional proteomics, depends on the availability of full-length cDNAs in appropriate expression-ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence to be cloned, which differentiates this method from the classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using two of the most popular recombinational cloning technologies, now commercially available from Invitrogen (Gateway) and BD Clontech (Creator).

Keywords:

  • Recombinational Cloning;
  • Gateway;
  • Creator