Unit

UNIT 3.20 Recombinational Cloning

  1. Jaehong Park,
  2. Joshua LaBaer

Published Online: 1 MAY 2006

DOI: 10.1002/0471142727.mb0320s74

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Park, J. and LaBaer, J. 2006. Recombinational Cloning. Current Protocols in Molecular Biology. 74:V:3.20:3.20.1–3.20.22.

Author Information

  1. Harvard Medical School, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2006
  2. Published Print: APR 2006

Abstract

The study of protein en masse, or functional proteomics, depends on the availability of full-length cDNAs in appropriate expression-ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence to be cloned, which differentiates this method from the classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using two of the most popular recombinational cloning technologies, now commercially available from Invitrogen (Gateway) and BD Clontech (Creator).

Keywords:

  • Recombinational Cloning;
  • Gateway;
  • Creator