UNIT 3.20 Recombinational Cloning
Published Online: 1 MAY 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Park, J. and LaBaer, J. 2006. Recombinational Cloning. Current Protocols in Molecular Biology. 74:V:3.20:3.20.1–3.20.22.
- Published Online: 1 MAY 2006
- Published Print: APR 2006
The study of protein en masse, or functional proteomics, depends on the availability of full-length cDNAs in appropriate expression-ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence to be cloned, which differentiates this method from the classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using two of the most popular recombinational cloning technologies, now commercially available from Invitrogen (Gateway) and BD Clontech (Creator).
- Recombinational Cloning;