Unit

UNIT 3.22 Gene and Genome Construction in Yeast

  1. Daniel G. Gibson

Published Online: 1 APR 2011

DOI: 10.1002/0471142727.mb0322s94

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Gibson, D. G. 2011. Gene and Genome Construction in Yeast. Current Protocols in Molecular Biology. 94:V:3.22:3.22.1–3.22.17.

Author Information

  1. The J. Craig Venter Institute, San Diego, California

Publication History

  1. Published Online: 1 APR 2011
  2. Published Print: APR 2011

Abstract

The yeast Saccharomyces cerevisiae has the capacity to take up and assemble dozens of different overlapping DNA molecules in one transformation event. These DNA molecules can be single-stranded oligonucleotides, to produce gene-sized fragments, or double-stranded DNA fragments, to produce molecules up to hundreds of kilobases in length, including complete bacterial genomes. This unit presents protocols for designing the DNA molecules to be assembled, transforming them into yeast, and confirming their assembly. Curr. Protoc. Mol. Biol. 94:3.22.1-3.22.17. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • DNA assembly;
  • DNA construction;
  • in vivo recombination;
  • yeast recombination;
  • yeast transformation;
  • gene construction;
  • genome construction;
  • synthetic biology