UNIT 4.2 Guanidine Methods for Total RNA Preparation

  1. Robert E. Kingston (CsCl isolation)1,
  2. Piotr Chomczynski (single-step isolation)2,
  3. Nicoletta Sacchi (single-step isolation)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0402s36

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Kingston, R. E., Chomczynski, P. and Sacchi, N. 2001. Guanidine Methods for Total RNA Preparation. Current Protocols in Molecular Biology. 36:I:4.2:4.2.1–4.2.9.

Author Information

  1. 1

    Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

  2. 2

    University of Cincinnati College of Medicine, Cincinnati, Ohio

  3. 3

    Laboratory of Molecular Oncology National Cancer Institute, Frederick, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1996


Three different methods for RNA preparation using guanidine are presented in this unit -- a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.