UNIT 4.2 Guanidine Methods for Total RNA Preparation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Kingston, R. E., Chomczynski, P. and Sacchi, N. 2001. Guanidine Methods for Total RNA Preparation. Current Protocols in Molecular Biology. 36:I:4.2:4.2.1–4.2.9.
- Published Online: 1 MAY 2001
- Published Print: OCT 1996
Three different methods for RNA preparation using guanidine are presented in this unit -- a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.