Unit

UNIT 4.3 Phenol/SDS Method for Plant RNA Preparation

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0403s09

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

2001. Phenol/SDS Method for Plant RNA Preparation. Current Protocols in Molecular Biology. 9:I:4.3:4.3.1–4.3.4.

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1990

Abstract

The method described here can be used to prepare RNA from a variety of eukaryotic tissues. The critical factor in isolating RNA from eukaryotic tissues is inactivating the endogenous RNase and preventing introduction of RNase from external sources. In general, protocols for making RNA from eukaryotic organisms involve lysing the cells in the presence of a strong denaturant and deproteinizing agent which inhibits RNase as well as strips the protein away from the RNA. In this protocol, the RNA is then separated from DNA and other impurities by selective precipitation in high salt.