UNIT 4.15 Synthesis and Labeling of RNA In Vitro
Published Online: 1 APR 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Huang, C. and Yu, Y.-T. 2013. Synthesis and Labeling of RNA In Vitro. Current Protocols in Molecular Biology. 102:II:4.15:4.15.1–4.15.14.
- Published Online: 1 APR 2013
- Published Print: APR 2013
This unit discusses several methods for generating large amounts of uniformly labeled, end-labeled, and site-specifically labeled RNAs in vitro. The methods involve a number of experimental procedures, including RNA transcription, 5′ dephosphorylation and rephosphorylation, 3′ terminal nucleotide addition (via ligation), site-specific RNase H cleavage directed by 2′-O-methyl RNA-DNA chimeras, and 2-piece splint ligation. The applications of these RNA radiolabeling approaches are also discussed. Curr. Protoc. Mol. Biol. 102:4.15.1–4.15.14. © 2013 by John Wiley & Sons, Inc.
- in vitro transcription;
- radiolabeled RNAs;
- RNA research