Unit

UNIT 4.16 RNA Isolation from Mammalian Samples

  1. Ximeng Liu,
  2. Shuko Harada

Published Online: 1 JUL 2013

DOI: 10.1002/0471142727.mb0416s103

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Liu, X. and Harada, S. 2013. RNA Isolation from Mammalian Samples. Current Protocols in Molecular Biology. 103:II:4.16:4.16.1–4.16.16.

Author Information

  1. Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 JUL 2013
  2. Published Print: JUL 2013

Abstract

RNA can be extracted from cultured cells, peripheral blood, bone marrow, plasma, serum, body fluids, and fresh or frozen tissues. RNA can also be extracted from formalin-fixed paraffin-embedded (FFPE) tissues. Methods for RNA extraction can be divided into three groups: phenol/chloroform extraction, silica spin-column absorption, and isopycnic gradient centrifugation. Two different RNA isolation procedures are described in this unit. The first basic protocol describes a one-step isolation involving monophasic lysis with guanidine isothiocyanate and phenol followed by chloroform extraction. The second basic protocol describes a silica-column separation method for RNA isolation. Curr. Protoc. Mol. Biol. 103:4.16.1–4.16.16. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • RNA isolation;
  • monophasic lysis reagent;
  • spin column