UNIT 4.16 RNA Isolation from Mammalian Samples
Published Online: 1 JUL 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Liu, X. and Harada, S. 2013. RNA Isolation from Mammalian Samples. Current Protocols in Molecular Biology. 103:II:4.16:4.16.1–4.16.16.
- Published Online: 1 JUL 2013
- Published Print: JUL 2013
RNA can be extracted from cultured cells, peripheral blood, bone marrow, plasma, serum, body fluids, and fresh or frozen tissues. RNA can also be extracted from formalin-fixed paraffin-embedded (FFPE) tissues. Methods for RNA extraction can be divided into three groups: phenol/chloroform extraction, silica spin-column absorption, and isopycnic gradient centrifugation. Two different RNA isolation procedures are described in this unit. The first basic protocol describes a one-step isolation involving monophasic lysis with guanidine isothiocyanate and phenol followed by chloroform extraction. The second basic protocol describes a silica-column separation method for RNA isolation. Curr. Protoc. Mol. Biol. 103:4.16.1–4.16.16. © 2013 by John Wiley & Sons, Inc.
- RNA isolation;
- monophasic lysis reagent;
- spin column