UNIT 4.17 Quantification of microRNA Expression with Next-Generation Sequencing
Published Online: 1 JUL 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Eminaga, S., Christodoulou, D. C., Vigneault, F., Church, G. M. and Seidman, J. 2013. Quantification of microRNA Expression with Next-Generation Sequencing. Current Protocols in Molecular Biology. 103:II:4.17:4.17.1–4.17.14.
- Published Online: 1 JUL 2013
- Published Print: JUL 2013
Rapid advancement of next-generation sequencing technologies has made it possible to study expression profiles of microRNAs (miRNAs) comprehensively and efficiently. Multiplexing miRNA libraries by barcoding can significantly reduce sequencing cost per sample without compromising library quality. This unit provides a step-by-step protocol for isolating miRNAs and constructing multiplexed miRNA libraries. Also described is a custom computational pipeline for analyzing the multiplexed miRNA library sequencing reads generated by Illumina-based technology. Curr. Protoc. Mol. Biol. 103:4.17.1–4.17.14. © 2013 by John Wiley & Sons, Inc.
- bioinformatics analysis