Unit

UNIT 4.19 Ribosomal RNA Depletion for Efficient Use of RNA-Seq Capacity

  1. Dominic O'Neil,
  2. Heike Glowatz,
  3. Martin Schlumpberger

Published Online: 1 JUL 2013

DOI: 10.1002/0471142727.mb0419s103

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

O'Neil, D., Glowatz, H. and Schlumpberger, M. 2013. Ribosomal RNA Depletion for Efficient Use of RNA-Seq Capacity. Current Protocols in Molecular Biology. 103:II:4.19:4.19.1–4.19.8.

Author Information

  1. Qiagen, Hilden, Germany

Publication History

  1. Published Online: 1 JUL 2013
  2. Published Print: JUL 2013

Abstract

Ribosomal RNA (rRNA) is the most highly abundant component of RNA, comprising the majority (>80% to 90%) of the molecules present in a total RNA sample. Depletion of this rRNA fraction is desirable prior to performing an RNA-seq reaction, so that sequencing capacity can be focused on more informative parts of the transcriptome. This unit describes an rRNA depletion method based on selective hybridization of oligonucleotides to rRNA, recognition with a hybrid-specific antibody, and removal of the antibody-hybrid complex on magnetic beads. Curr. Protoc. Mol. Biol. 103:4.19.1–4.19.8. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • rRNA depletion;
  • sample preparation;
  • RNA-seq;
  • next generation sequencing;
  • transcriptome