Unit

UNIT 4.20 Purification of Radiolabeled RNA Products Using Denaturing Gel Electrophoresis

  1. Hironori Adachi,
  2. Yi-Tao Yu

Published Online: 6 JAN 2014

DOI: 10.1002/0471142727.mb0420s105

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Adachi, H. and Yu, Y.-T. 2014. Purification of Radiolabeled RNA Products Using Denaturing Gel Electrophoresis. Current Protocols in Molecular Biology. 105:II:4.20:4.20.1–4.20.13.

Author Information

  1. Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester Medical Center, Rochester, New York

Publication History

  1. Published Online: 6 JAN 2014

Abstract

This unit discusses a basic method for purification of radiolabeled RNAs using denaturing polyacrylamide gel electrophoresis. The method consists of a number of experimental procedures, including total RNA preparation from yeast cells, isolation of a specific RNA from total yeast RNA, RNA 3′-terminal labeling using nucleotide (5′ [32P]pCp) addition (via ligation), denaturing (8 M urea) polyacrylamide gel electrophoresis, and RNA extraction from the gel slice. Key points for achieving good electrophoretic separation of RNA are also discussed. Curr. Protoc. Mol. Biol. 105:4.20.1-4.20.13. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • RNA purification;
  • radiolabeled RNAs;
  • denaturing polyacrylamide electrophoresis (PAGE);
  • yeast cells;
  • RNA molecular biology