UNIT 4.20 Purification of Radiolabeled RNA Products Using Denaturing Gel Electrophoresis
Published Online: 6 JAN 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Adachi, H. and Yu, Y.-T. 2014. Purification of Radiolabeled RNA Products Using Denaturing Gel Electrophoresis. Current Protocols in Molecular Biology. 105:II:4.20:4.20.1–4.20.13.
- Published Online: 6 JAN 2014
This unit discusses a basic method for purification of radiolabeled RNAs using denaturing polyacrylamide gel electrophoresis. The method consists of a number of experimental procedures, including total RNA preparation from yeast cells, isolation of a specific RNA from total yeast RNA, RNA 3′-terminal labeling using nucleotide (5′ [32P]pCp) addition (via ligation), denaturing (8 M urea) polyacrylamide gel electrophoresis, and RNA extraction from the gel slice. Key points for achieving good electrophoretic separation of RNA are also discussed. Curr. Protoc. Mol. Biol. 105:4.20.1-4.20.13. © 2014 by John Wiley & Sons, Inc.
- RNA purification;
- radiolabeled RNAs;
- denaturing polyacrylamide electrophoresis (PAGE);
- yeast cells;
- RNA molecular biology