UNIT 4.22 Preparation of Single-Cell RNA-Seq Libraries for Next Generation Sequencing
Published Online: 1 JUL 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Trombetta, J. J., Gennert, D., Lu, D., Satija, R., Shalek, A. K. and Regev, A. 2014. Preparation of Single-Cell RNA-Seq Libraries for Next Generation Sequencing. Current Protocols in Molecular Biology. 107:II:4.22:4.22.1–4.22.17.
- Published Online: 1 JUL 2014
For the past several decades, due to technical limitations, the field of transcriptomics has focused on population-level measurements that can mask significant differences between individual cells. With the advent of single-cell RNA-Seq, it is now possible to profile the responses of individual cells at unprecedented depth and thereby uncover, transcriptome-wide, the heterogeneity that exists within these populations. This unit describes a method that merges several important technologies to produce, in high-throughput, single-cell RNA-Seq libraries. Complementary DNA (cDNA) is made from full-length mRNA transcripts using a reverse transcriptase that has terminal transferase activity. This, when combined with a second “template-switch” primer, allows for cDNAs to be constructed that have two universal priming sequences. Following preamplification from these common sequences, Nextera XT is used to prepare a pool of 96 uniquely indexed samples ready for Illumina sequencing. Curr. Protoc. Mol. Biol. 107:4.22.1-4.22.17. © 2014 by John Wiley & Sons, Inc.
- single-cell RNA-Seq;
- cell type;
- single cell;
- low-input RNA-Seq;
- Next-generation sequencing;