Unit

UNIT 5.1 Genomic DNA Libraries

  1. David D. Moore

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0501s00

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Moore, D. D. 2001. Genomic DNA Libraries. Current Protocols in Molecular Biology. 00:I:5.1:5.1.1–5.1.3.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1987

Abstract

Genomic DNA libraries are almost always screened by hybridization using a radioactive nucleic acid probe. Since this approach is essentially independent of a particular vector or type of target DNA, the main problem faced when considering creation of a genomic DNA library is simply generating a large enough number of recombinant DNA clones. The basic strategies used to address this problem have included both minimizing the number of clones necessary by incorporating large fragments of genomic DNA, and maximizing cloning efficiency by using vectors based on bacteriophage l. This unit discusses the appropriate numerical considerations for both ordinary genomic DNA libraries and subgenomic DNA libraries, and then describes a limited number of appropriate vectors.