Unit

UNIT 5.2 cDNA Libraries

  1. David D. Moore

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0502s01

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Moore, D. D. 2001. cDNA Libraries. Current Protocols in Molecular Biology. 1:I:5.2:5.2.1.

Author Information

  1. Massachusetts General Hospital And Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1988

Abstract

This unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double-stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be necessary to include the clone of interest, and choices of linkers or adaptors for insertion into the vector.