UNIT 5.2 cDNA Libraries
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Moore, D. D. 2001. cDNA Libraries. Current Protocols in Molecular Biology. 1:I:5.2:5.2.1.
- Published Online: 1 MAY 2001
- Published Print: JAN 1988
This unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double-stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be necessary to include the clone of interest, and choices of linkers or adaptors for insertion into the vector.