UNIT 5.3 Size Fractionation Using Sucrose Gradients
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Weis, J. H. and Quertermous, T. 2001. Size Fractionation Using Sucrose Gradients. Current Protocols in Molecular Biology. 00:II:5.3:5.3.1–5.3.8.
- Published Online: 1 MAY 2001
- Published Print: OCT 1987
DNA fragments migrate through a linear sucrose gradient at a rate that is dependent on their size. This procedure described in this unit provides good resolution for DNA fragments 5 to 60 kb in size, and sucrose gradients are also useful for purification of bacteriophage l vector arms. Partially digested genomic DNA can be fractionated for the production of cosmid or bacteriophage libraries and completely digested DNA can be fractionated for subgenomic DNA libraries. Protocols are provided in this unit for both partial and complete enzyme digestion of genomic DNA.