Unit

UNIT 5.8A Production of a Complete cDNA Library

  1. Lloyd B. Klickstein

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0508as18

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Klickstein, L. B. 2001. Production of a Complete cDNA Library. Current Protocols in Molecular Biology. 18:5.8.1–5.8.8.

Author Information

  1. Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1992

Abstract

A complete cDNA library is one that contains at least one cDNA clone representing each mRNA in the cell. A method is provided in this unit for calculating the required base of a cDNA library such that the library should contain at least one copy of every mRNA. Either a phage or plasmid vector can be used in constructing a complete cDNA library, and protocols are described for both. Procedures for evaluating these cDNA libraries are also provided along with a procedure for evaluating the quality of the λ arms used in constructing complete phage cDNA libraries.