UNIT 5.8A Production of a Complete cDNA Library
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Klickstein, L. B. 2001. Production of a Complete cDNA Library. Current Protocols in Molecular Biology. 18:IV:5.8A:5.8.1–5.8.8.
- Published Online: 1 MAY 2001
- Published Print: APR 1992
A complete cDNA library is one that contains at least one cDNA clone representing each mRNA in the cell. A method is provided in this unit for calculating the required base of a cDNA library such that the library should contain at least one copy of every mRNA. Either a phage or plasmid vector can be used in constructing a complete cDNA library, and protocols are described for both. Procedures for evaluating these cDNA libraries are also provided along with a procedure for evaluating the quality of the λ arms used in constructing complete phage cDNA libraries.