Unit

UNIT 5.10 Amplification of a Bacteriophage Library

  1. Lloyd B. Klickstein

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0510s34

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Klickstein, L. B. 2001. Amplification of a Bacteriophage Library. Current Protocols in Molecular Biology. 34:V:5.10:5.10.1–5.10.3.

Author Information

  1. Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1996

Abstract

This protocol may be used for genomic DNA or cDNA libraries. A freshly packaged and titered library is adsorbed to log phase plating bacteria. The mixture is then plated at high density and allowed to grow until the plaques are just subconfluent. The phage are eluted from the plate by overnight incubation with phage buffer and the library is titered and stored.