UNIT 5.10 Amplification of a Bacteriophage Library
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Klickstein, L. B. 2001. Amplification of a Bacteriophage Library. Current Protocols in Molecular Biology. 34:V:5.10:5.10.1–5.10.3.
- Published Online: 1 MAY 2001
- Published Print: APR 1996
This protocol may be used for genomic DNA or cDNA libraries. A freshly packaged and titered library is adsorbed to log phase plating bacteria. The mixture is then plated at high density and allowed to grow until the plaques are just subconfluent. The phage are eluted from the plate by overnight incubation with phage buffer and the library is titered and stored.