UNIT 5.11 Amplification of Cosmid and Plasmid Libraries
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Weis, J. H. 2001. Amplification of Cosmid and Plasmid Libraries. Current Protocols in Molecular Biology. 34:V:5.11:5.11.1–5.11.2.
- Published Online: 1 MAY 2001
- Published Print: APR 1996
The purpose of amplification is to provide a reagent library that can be used many times. In this protocol, cacteria containing the recombinant clones are grown on agar plates, washed off the plates, and stored in glycerol. An amplified cosmid or plasmid library will contain one library's equivalence in 10 to 100 µl. By producing 100 µl of such a library frozen in 100 1-ml tubes, over 1000 platings can be expected.