Unit

UNIT 5.11 Amplification of Cosmid and Plasmid Libraries

  1. John H. Weis

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0511s34

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Weis, J. H. 2001. Amplification of Cosmid and Plasmid Libraries. Current Protocols in Molecular Biology. 34:V:5.11:5.11.1–5.11.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1996

Abstract

The purpose of amplification is to provide a reagent library that can be used many times. In this protocol, cacteria containing the recombinant clones are grown on agar plates, washed off the plates, and stored in glycerol. An amplified cosmid or plasmid library will contain one library's equivalence in 10 to 100 µl. By producing 100 µl of such a library frozen in 100 1-ml tubes, over 1000 platings can be expected.