UNIT 6.7 Immunoscreening of Fusion Proteins Produced in Lambda Plaques
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
St. John, T. P. 2001. Immunoscreening of Fusion Proteins Produced in Lambda Plaques. Current Protocols in Molecular Biology. 17:IV:6.7:6.7.1–6.7.6.
- Published Online: 1 MAY 2001
- Published Print: JAN 1990
Screening large numbers of plaques containing particular proteins is accomplished by techniques that are analogous to those described for screening with radioactive DNA probes. However, in the basic protocol described here, the plaques are screened with antibodies specific to the desired proteins. An alternate protocol provides a method for increasing the amount of recombinant protein in each plaque by inducing expression from the lac promoter that directs its expression.