Unit

UNIT 6.7 Immunoscreening of Fusion Proteins Produced in Lambda Plaques

  1. Thomas P. St. John

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0607s09

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

St. John, T. P. 2001. Immunoscreening of Fusion Proteins Produced in Lambda Plaques. Current Protocols in Molecular Biology. 17:IV:6.7:6.7.1–6.7.6.

Author Information

  1. Fred Hutchinson Cancer Research Center, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1990

Abstract

Screening large numbers of plaques containing particular proteins is accomplished by techniques that are analogous to those described for screening with radioactive DNA probes. However, in the basic protocol described here, the plaques are screened with antibodies specific to the desired proteins. An alternate protocol provides a method for increasing the amount of recombinant protein in each plaque by inducing expression from the lac promoter that directs its expression.