UNIT 6.8 Immunoscreening after Hybrid Selection and Translation

  1. Baruch Velan

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0608s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Velan, B. 2001. Immunoscreening after Hybrid Selection and Translation. Current Protocols in Molecular Biology. 24:IV:6.8:6.8.1–6.8.5.

Author Information

  1. Israel Institute for Biological Research, Ness Ziona, Israel

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1991


In this procedure, plasmid cDNA clones are screened for their ability to select a particular mRNA. Plasmid DNA is bound to nitrocellulose filters, hybridized to mRNA, washed, and the selected mRNA is eluted from the filter. Eluted mRNA is characterized by translation into 35S-labeled protein, which is identified by immunoprecipitation and denaturing (SDS) polyacrylamide gel electrophoresis. The desired clone is that which is able to select an mRNA that translates into the desired protein. This procedure can be modified to characterize cosmids and bacteriophage DNA.