Unit

UNIT 6.9 Overview of Strategies for Screening YAC Libraries and Analyzing YAC Clones

  1. David D. Chaplin,
  2. Bernard H. Brownstein

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0609s20

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Chaplin, D. D. and Brownstein, B. H. 2001. Overview of Strategies for Screening YAC Libraries and Analyzing YAC Clones. Current Protocols in Molecular Biology. 20:V:6.9:6.9.1–6.9.7.

Author Information

  1. Howard Hughes Medical Institute and Washington University School of Medicine, St. Louis, Missouri

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1992

Abstract

This unit provides an introduction to the use of yeast artificial chromosome-bearing yeast clones (hereafter referred to as YAC clones) in genome analysis. It describes criteria for designing a polymerase chain reaction (PCR) assay to be used in screening a YAC core library and discusses the rationale for verification and characterization of YAC clones obtained from these core laboratories. Protocols for maintaining YAC clones, analyzing YAC insert structure, preparing YAC DNA, and subcloning YAC inserts into other vectors are presented elsewhere in this volume.