Chapter Introduction

Introduction and Historical Overview of DNA Sequencing

  1. F. Kenneth Nelson1,
  2. Michael Snyder2,
  3. Andrew F. Gardner3,
  4. Cynthia L. Hendrickson4,
  5. Jay A. Shendure5,
  6. Gregory J. Porreca6,
  7. George M. Church7,
  8. Frederick M. Ausubel8,
  9. Jingyue Ju9,
  10. Jan Kieleczawa10,
  11. Barton E. Slatko3

Published Online: 1 OCT 2011

DOI: 10.1002/0471142727.mb0700s96

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Kenneth Nelson, F., Snyder, M., Gardner, A. F., Hendrickson, C. L., Shendure, J. A., Porreca, G. J., Church, G. M., Ausubel, F. M., Ju, J., Kieleczawa, J. and Slatko, B. E. 2011. Introduction and Historical Overview of DNA Sequencing. Current Protocols in Molecular Biology. 96:7.0:7.0.1–7.0.18.

Author Information

  1. 1

    Yale University MCDB, New Haven, Connecticut

  2. 2

    Department of Genetics Stanford University , Stanford, California

  3. 3

    New England Biolabs, Ipswich, Massachusetts

  4. 4

    HudsonAlpha Institute for Biotechnology , Huntsville, Alabama

  5. 5

    Department of Genome Sciences, University of Washington, Seattle, Washington

  6. 6

    Good Start Genetics, Inc., Cambridge, Massachusetts

  7. 7

    Department of Genetics, Harvard Medical School, Boston, Massachusetts

  8. 8

    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts

  9. 9

    Center for Genome Technology & Biomolecular Engineering, Columbia University, New York, New York

  10. 10

    Wyzer Biosciences, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011


The process of DNA sequencing has made tremendous strides in throughput, improved accuracy, ease of production, and lowered cost. As the practice of DNA sequencing has improved, so has the downstream data analysis with sophisticated databases and bioinformatics tools. Together, these advances have enlarged the number of applications upon which DNA sequencing can be brought to bear. This introductory unit provides a description of DNA sequencing with a focus on current and “NextGen” (second and third generation) automated technologies and applications. Curr. Protoc. Mol. Biol. 96:7.0.1-7.0.18. © 2011 by John Wiley & Sons, Inc.


  • NextGen DNA sequencing;
  • high-throughput sequencing;
  • epigenomics;
  • transcriptome;
  • ChIP Seq;
  • CNV;
  • copy number variation