UNIT 7.1 DNA Sequencing Strategies
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Slatko, B. E., Eckert, R. L., Albright, L. M. and Ausubel, F. M. 2001. DNA Sequencing Strategies. Current Protocols in Molecular Biology.
- Published Online: 1 MAY 2001
- Published Print: APR 1999
This is not the most recent version of the article. View current version (1 OCT 2011)
This unit contains a general discussion of factors that should be considered before embarking on a DNA sequencing project. In general, any sequencing strategy should include plans for sequencing both strands of the DNA fragment. Complementary strand confirmation leads to higher accuracy, especially when sequencing regions where artifacts such as “compressions” are a problem. Sequencing the opposite strand is often required to obtain accurate data for such regions. The most commonly used methods for generating appropriately sized DNA fragments for dideoxy and chemical sequencing are discussed in this unit, and the biochemistry underlying these procedures, as well as how to choose between these and alternative sequencing methods, are discussed in the introduction to this chapter.