UNIT 7.1 Overview of DNA Sequencing Strategies
Published Online: 1 JAN 2008
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Shendure, J. A., Porreca, G. J. and Church, G. M. 2008. Overview of DNA Sequencing Strategies. Current Protocols in Molecular Biology. 81:7.1:7.1.1–7.1.11.
- Published Online: 1 JAN 2008
- Published Print: JAN 2008
This is not the most recent version of the article. View current version (1 OCT 2011)
Efficient and cost-effective DNA sequencing technologies have been, and may continue to be, critical to the progress of molecular biology. This overview of DNA sequencing strategies provides a high-level review of six distinct approaches to DNA sequencing: (a) dideoxy sequencing; (b) cyclic array sequencing; (c) sequencing-by-hybridization; (d) microelectrophoresis; (e) mass spectrometry; and (f) nanopore sequencing. The primary focus is on dideoxy sequencing, which has been the dominant technology since 1977, and on cyclic array strategies, for which several competitive implementations have been developed since 2005. Because the field of DNA sequencing is changing rapidly, this unit represents a snapshot of this particular moment. Curr. Protoc. Mol. Biol. 81:7.1.1-7.1.11. © 2008 by John Wiley & Sons, Inc.