UNIT 7.1 Overview of DNA Sequencing Strategies
Published Online: 1 OCT 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Shendure, J. A., Porreca, G. J., Church, G. M., Gardner, A. F., Hendrickson, C. L., Kieleczawa, J. and Slatko, B. E. 2011. Overview of DNA Sequencing Strategies. Current Protocols in Molecular Biology. 96:7.1:7.1.1–7.1.23.
- Published Online: 1 OCT 2011
- Published Print: OCT 2011
Efficient and cost-effective DNA sequencing technologies are critical to the progress of molecular biology. This overview of DNA sequencing strategies provides a high-level review of seven distinct approaches to DNA sequencing: (a) dideoxy sequencing; (b) solid phase sequencing; (c) sequencing-by-hybridization; (d) mass spectrometry; (e) cyclic array sequencing; (f) microelectrophoresis; and (g) nanopore sequencing. Other platforms currently in development are also briefly described. The primary focus here is on Sanger dideoxy sequencing, which has been the dominant technology since 1977, and on cyclic array strategies, for which several competitive implementations have been developed since 2005. Because the field of DNA sequencing is changing rapidly, this unit represents a snapshot as of September, 2011. Curr. Protoc. Mol. Biol. 96:7.1.1-7.1.23. © 2011 by John Wiley & Sons, Inc.
- DNA sequencing;
- next-generation sequencing;
- cyclic array sequencing;
- sequencing by ligation;
- nanopore sequencing