UNIT 7.1 Overview of DNA Sequencing Strategies

  1. Jay A. Shendure1,
  2. Gregory J. Porreca2,
  3. George M. Church3,
  4. Andrew F. Gardner4,
  5. Cynthia L. Hendrickson5,
  6. Jan Kieleczawa6,
  7. Barton E. Slatko4

Published Online: 1 OCT 2011

DOI: 10.1002/0471142727.mb0701s96

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Shendure, J. A., Porreca, G. J., Church, G. M., Gardner, A. F., Hendrickson, C. L., Kieleczawa, J. and Slatko, B. E. 2011. Overview of DNA Sequencing Strategies. Current Protocols in Molecular Biology. 96:7.1:7.1.1–7.1.23.

Author Information

  1. 1

    Department of Genome Sciences, University of Washington, Seattle, Washington

  2. 2

    Good Start Genetics, Inc., Cambridge, Massachusetts

  3. 3

    Department of Genetics, Harvard Medical School, Boston, Massachusetts

  4. 4

    New England Biolabs, Ipswich, Massachusetts

  5. 5

    HudsonAlpha Institute for Biotechnology, Huntsville, Alabama

  6. 6

    Wyzer Biosciences, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011


Efficient and cost-effective DNA sequencing technologies are critical to the progress of molecular biology. This overview of DNA sequencing strategies provides a high-level review of seven distinct approaches to DNA sequencing: (a) dideoxy sequencing; (b) solid phase sequencing; (c) sequencing-by-hybridization; (d) mass spectrometry; (e) cyclic array sequencing; (f) microelectrophoresis; and (g) nanopore sequencing. Other platforms currently in development are also briefly described. The primary focus here is on Sanger dideoxy sequencing, which has been the dominant technology since 1977, and on cyclic array strategies, for which several competitive implementations have been developed since 2005. Because the field of DNA sequencing is changing rapidly, this unit represents a snapshot as of September, 2011. Curr. Protoc. Mol. Biol. 96:7.1.1-7.1.23. © 2011 by John Wiley & Sons, Inc.


  • DNA sequencing;
  • next-generation sequencing;
  • polony;
  • cyclic array sequencing;
  • genomics;
  • sequencing by ligation;
  • nanopore sequencing