Unit

UNIT 7.2 “First Generation” Automated DNA Sequencing Technology

  1. Barton E. Slatko1,
  2. Jan Kieleczawa2,
  3. Jingyue Ju3,
  4. Andrew F. Gardner1,
  5. Cynthia L. Hendrickson4,
  6. Frederick M. Ausubel5

Published Online: 1 OCT 2011

DOI: 10.1002/0471142727.mb0702s96

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Slatko, B. E., Kieleczawa, J., Ju, J., Gardner, A. F., Hendrickson, C. L. and Ausubel, F. M. 2011. “First Generation” Automated DNA Sequencing Technology. Current Protocols in Molecular Biology. 96:7.2:7.2.1–7.2.28.

Author Information

  1. 1

    New England Biolabs, Inc., Ipswich, Massachusetts

  2. 2

    Wyzer Biosciences , Cambridge, Massachusetts

  3. 3

    Center for Genome Technology & Biomolecular Engineering, Columbia University , New York, New York

  4. 4

    HudsonAlpha Institute for Biotechnology , Huntsville, Alabama

  5. 5

    Department of Molecular Biology, Massachusetts General Hospital , Boston, Massachusetts

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011

Abstract

Beginning in the 1980s, automation of DNA sequencing has greatly increased throughput, reduced costs, and enabled large projects to be completed more easily. The development of automation technology paralleled the development of other aspects of DNA sequencing: better enzymes and chemistry, separation and imaging technology, sequencing protocols, robotics, and computational advancements (including base-calling algorithms with quality scores, database developments, and sequence analysis programs). Despite the emergence of high-throughput sequencing platforms, automated Sanger sequencing technology remains useful for many applications. This unit provides background and a description of the “First-Generation” automated DNA sequencing technology. It also includes protocols for using the current Applied Biosystems (ABI) automated DNA sequencing machines. Curr. Protoc. Mol. Biol. 96:7.2.1-7.2.28. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • dideoxy DNA sequencing;
  • thermal cycle sequencing;
  • BigDye terminator;
  • high-throughput DNA sequencing;
  • genomics