UNIT 7.7 Computer Manipulation of DNA and Protein Sequences

  1. J. Michael Cherry

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0707s30

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Cherry, J. M. 2001. Computer Manipulation of DNA and Protein Sequences. Current Protocols in Molecular Biology. 30:7.7:7.7.1–7.7.23.

Author Information

  1. Stanford University, Palo Alto, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1995


This unit outlines a variety of methods by which DNA sequences can be manipulated by computers. Procedures for entering sequence data into the computer and assembling raw sequence data into a contiguous sequence are described first, followed by a description of methods of analyzing and manipulating sequences--e.g., verifying sequences, constructing restriction maps, designing oligonucleotides, identifying protein-coding regions, and predicting secondary structures. This unit also provides information on the large amount of software available for sequence analysis.The appendix to this unit lists some of the commercial software, shareware, and free software related to DNA sequence manipulation. The goal of this unit is to serve as a starting point for researchers interested in utilizing the tremendous sequencing resources available to the computer-knowledgeable molecular biology laboratory.