UNIT 7.8 Polony DNA Sequencing

  1. Gregory J. Porreca,
  2. Jay Shendure,
  3. George M. Church

Published Online: 1 NOV 2006

DOI: 10.1002/0471142727.mb0708s76

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Porreca, G. J., Shendure, J. and Church, G. M. 2006. Polony DNA Sequencing. Current Protocols in Molecular Biology. 76:7.8:7.8.1–7.8.22.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2006
  2. Published Print: OCT 2006


Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.


  • DNA sequencing;
  • high throughput;
  • polony sequencing;
  • resequencing;
  • sequencing by synthesis