UNIT 7.9 Bisulfite Sequencing of DNA
Published Online: 1 JUL 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Darst, R. P., Pardo, C. E., Ai, L., Brown, K. D. and Kladde, M. P. 2010. Bisulfite Sequencing of DNA. Current Protocols in Molecular Biology. 91:7.9:7.9.1–7.9.17.
- Published Online: 1 JUL 2010
- Published Print: JUL 2010
Exact positions of 5-methylcytosine (m5C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation. Amplifying regions of interest from deaminated DNA and sequencing products cloned from amplicons permits determination of methylation at single-nucleotide resolution along single DNA molecules, which is not possible with other methylation analysis techniques. This unit describes a BGS technique suitable for most DNA sources, including formaldehyde-fixed tissue. Considerations for experimental design and common sources of error are discussed. Curr. Protoc. Mol. Biol. 91:7.9.1-7.9.17. © 2010 by John Wiley & Sons, Inc.
- DNA methylation;