UNIT 7.9 Bisulfite Sequencing of DNA

  1. Russell P. Darst,
  2. Carolina E. Pardo,
  3. Lingbao Ai,
  4. Kevin D. Brown,
  5. Michael P. Kladde

Published Online: 1 JUL 2010

DOI: 10.1002/0471142727.mb0709s91

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Darst, R. P., Pardo, C. E., Ai, L., Brown, K. D. and Kladde, M. P. 2010. Bisulfite Sequencing of DNA. Current Protocols in Molecular Biology. 91:7.9:7.9.1–7.9.17.

Author Information

  1. University of Florida College of Medicine, Gainesville, Florida

Publication History

  1. Published Online: 1 JUL 2010
  2. Published Print: JUL 2010


Exact positions of 5-methylcytosine (m5C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation. Amplifying regions of interest from deaminated DNA and sequencing products cloned from amplicons permits determination of methylation at single-nucleotide resolution along single DNA molecules, which is not possible with other methylation analysis techniques. This unit describes a BGS technique suitable for most DNA sources, including formaldehyde-fixed tissue. Considerations for experimental design and common sources of error are discussed. Curr. Protoc. Mol. Biol. 91:7.9.1-7.9.17. © 2010 by John Wiley & Sons, Inc.


  • DNA methylation;
  • epigenetics;
  • chromatin